TABLE 1:
Global protein levels for Rng10 and its truncations along with known proteins for the standard curve measured by quantitative fluorescence microscopy.
| Protein | Number of cells analyzed | Intensity (mean ± SD) | Molecules per cell (mean ± SD) | Global concentration (μM)a |
|---|---|---|---|---|
| Rng10 | 58 | 1230 ± 300 | 6180 ± 1520 | 0.38 ± 0.09 |
| 62 | 2600 ± 300b | |||
| Rng10(1-200) | 60 | 1780 ± 270 | 11,940 ± 1780 | 0.74 ± 0.11 |
| Rng10(1-450) | 60 | 1340 ± 230 | 7280 ± 1240 | 0.45 ± 0.08 |
| Rng10(1-750) | 58 | 1210 ± 210 | 5960 ± 1020 | 0.37 ± 0.06 |
| Rng10(201-1038) | 51 | 2285 ± 240b | 4620 ± 480 | 0.29 ± 0.03 |
| Rng10(451-1038) | 65 | 2470 ± 220b | 5570 ± 490 | 0.35 ± 0.03 |
| Rng10(751-1038) | 63 | 2290 ± 260b | 4650 ± 530 | 0.29 ± 0.03 |
| Rng10(201-750) | 82 | 3191 ± 360b | 9150 ± 1030 | 0.57 ± 0.06 |
| Myo2 | 64 | 1060 ± 500 | 7300 ± 3450c | 0.45 ± 0.21 |
| Rlc1 | 64 | 1720 ± 230 | 9600 ± 1280c | 0.60 ± 0.08 |
| Spn1 | 61 | 1760 ± 280 | 10,300 ± 1620c | 0.63 ± 0.10 |
| Cdc15 | 64 | 4010 ± 600 | 35,600 ± 5370c | 2.13 ± 0.33 |
aThe global concentration was calculated with a mean cytoplasm volume of 27 μm3, which was derived from a mean cell volume of 92 μm3 and the cytoplasm occupying ∼29% of the cell volume (Wu and Pollard, 2005).
bIntensity for proteins tagged with mECitrine. Other intensities are from the mEGFP tag.
cThe mean numbers are from published data (Wu and Pollard, 2005).