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. 2016 Aug 15;27(16):2542–2553. doi: 10.1091/mbc.E16-03-0163

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© 2016 Li, Miao, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — α-SNAP regulates functional assembly and calcium selectivity of Orai1 oligomers. (A) Representative Western blot showing depletion of α-SNAP in siRNA-treated cells. (B) Representative Fura-2 imaging plot showing defective SOCE in α-SNAP siRNA–treated cells. (C) Histogram of mEos-Orai1 bleaching steps obtained from siRNA-treated Orai1^−/− MEFs expressing mEos-Orai1 and Stim1-myc (500–700 spots). (D) Binomial fitting of experimental data (red bars) from C to various models (colored bars) from Table 3. (E) Western blot showing chemically cross-linked Orai1 oligomers from SCR and α-SNAP siRNA–treated HEK 293 cells with stable and inducible expression of flag-Orai1 and Stim1-myc. (F) Representative SBFI imaging plot showing store-operated sodium influx in SCR or α-SNAP siRNA–treated WT or Orai1^−/− MEF cells.