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. 2016 Aug 15;27(16):2542–2553. doi: 10.1091/mbc.E16-03-0163

FIGURE 2:

FIGURE 2:

α-SNAP regulates functional assembly and calcium selectivity of Orai1 oligomers. (A) Representative Western blot showing depletion of α-SNAP in siRNA-treated cells. (B) Representative Fura-2 imaging plot showing defective SOCE in α-SNAP siRNA–treated cells. (C) Histogram of mEos-Orai1 bleaching steps obtained from siRNA-treated Orai1−/− MEFs expressing mEos-Orai1 and Stim1-myc (500–700 spots). (D) Binomial fitting of experimental data (red bars) from C to various models (colored bars) from Table 3. (E) Western blot showing chemically cross-linked Orai1 oligomers from SCR and α-SNAP siRNA–treated HEK 293 cells with stable and inducible expression of flag-Orai1 and Stim1-myc. (F) Representative SBFI imaging plot showing store-operated sodium influx in SCR or α-SNAP siRNA–treated WT or Orai1−/− MEF cells.