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. 2016 Aug 15;27(16):2565–2575. doi: 10.1091/mbc.E16-03-0200

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© 2016 Ly, Moroz, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — Fluorescence recovery after photobleaching of GFP-Lmod2 and GFP-Lmod2 (W73D). (A) Mean relative recovery after photobleaching over time for GFP-Lmod2 (blue triangles) and GFP-Lmod2 (W73D) (red squares). (B) Recovery data fit using nonlinear regression curves with the single-exponential association equation R = M[1 − exp(−kt)] for each individual cell, where R is the relative recovery of fluorescence at time t. Mean mobile fraction (M), rate (k), and half-time of recovery (t_1/2) are indicated ± SEM. Bold indicates values that are statistically significantly different between GFPLmod2 and GFPLmod2 (W73D). n = 34–36 from three independent cultures; *p < 0.05, Student’s t test.