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. 2016 Aug 15;27(16):2598–2612. doi: 10.1091/mbc.E16-01-0049

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© 2016 Mellado et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — Intracellular localization of SltA. HA₃ fusions were detected using a secondary antibody labeled with Alexa Fluor 488 (Alexa 488). (A) Immunofluorescence detection of SltA-HA₃ expressed by strain MAD3652. (B) Detection of SltA⁴⁰⁰-HA₃ expressed by null sltB strain MAD3693 under the regulation of the thiamine-repressible promoter in the absence of thiamine. (C) Detection of HypA-HA₃ fusion as a quality control for immunodetection procedure. HypA-HA₃ (MAD4784) displayed a similar localization to HypA-GFP, as described in Pinar et al. (2015). The red asterisk indicates the Spitzenkörper, and blue arrowheads indicate the intracellular vesicles, probably the Golgi. (D) Detection of SltA400-HA3 expressed by strain MAD4661 under the regulation of the thiamine-repressible promoter in the absence of thiamine. Bar, 5 μm.