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. Author manuscript; available in PMC: 2017 Aug 11.
Published in final edited form as: Cell. 2016 Jul 21;166(4):841–854. doi: 10.1016/j.cell.2016.06.040

Figure 5. Reduced oxidative respiration and complex activity in mitochondria of IL-33 deficient BAT.

Figure 5

(A, B) Representative recording of oxygen consumption by mitochondria purified from BAT of ~6-week-old WT and Il33−/− mice housed at 22°C (n=3-4 per genotype). (C) Immunoblotting for components of mitochondrial respiratory chain, VDAC1 and Cytochrome C in purified mitochondria from BAT of WT and Il33−/− mice housed at 22°C (n=3 per genotype). (D-G) Quantification of mitochondrial respiratory chain activities using mitochondria purified from BAT of ~6-week-old WT and Il33−/− mice housed at 22°C (n=3 per genotype). Complex I (D), complex II (E), complex III (F) and complex IV (G). (H-K) Representative transmission electron micrographs of BAT from ~5-week-old WT and Il33−/− mice housed at 22°C and 5°C, magnification 4000x. Data are represented as mean ± SEM. See also Figure S5.