FIG. 1.

hDPC-seeded E1001(1k)/β-TCP scaffolds. hDPC-seeded and acellular scaffolds were cultured in osteogenic media for 1 week before implantation. Cytoskeletal F-Actin was stained using Alexa Fluor 594-conjugated phalloidin (red). Nuclei were stained with Hoechst 33258 (blue). E1001(1k)/β-TCP scaffolds exhibited autofluorescence under GFP filter (green). (A) Scaffold seeded with high cell density. (B) Scaffold seeded with low cell density. (C) Acellular scaffold. (A′–C′) show high magnification of corresponding boxed areas. Scale bars = 500 μm (A–C) and 20 μm (A′–C′). hDPC, human dental pulp cell. Color images available online at www.liebertpub.com/tea