FIG. 5.

Immunofluorescent analyses of rat mandibular defect healing. Positive immunostaining is indicated in red, E1001(1k)-bTCP scaffold autofluorescence in green. (A) OC staining. (A1′–A4′) Higher magnification images of the correlated boxed area of (A1–A4). (A1, A1′) Cell-high density-6W; (A2, A2′) cell-low density-6W; (A3, A3′) scaffold alone-6W; (A4, A4′) BMP-6W; yellow dots outline the border of scaffolding area. (B) DSPP staining; (B1) cell-high density-6W; (B2) cell-low density-6W; (B3) scaffold alone-6W; (B4) BMP-6W. (C) Human mitochondria staining; (C1) cell-high density-3W; (C2) cell-low density-3W; (C3) scaffold alone-3W. Scale bar = 500 μm (A1–A4), 50 μm (B1–B4), 20 μm (A1′–A4′, C1–C3). DSPP, dentin sialophosphoprotein; SC, scaffold; nb, normal bone. Color images available online at www.liebertpub.com/tea