Figure 11. FRAP-like FDS-SV from mixtures of rsEGFP2-GluA3 and DL488-GluA2.
Equimolar mixtures at 0.1 nM (a,c), 2 nM (b,d), 3nM (e,g) and 10 nM (f,h) were sedimented at 50,000 rpm, 20°C, with initial 20 min stationary exposure at 6.5 cm of the excitation beam at 488 nm, prior to radial scanning with 488 nm excitation (every second scan shown). Initial exposure parameters were taken from the rsEGFP2-GluA3 data in Figure 2f and fixed. Solid lines and residuals are from the MCMC decomposition (a,b,e,f) with resulting distributions shown below the scans in panels (c,d,g,h), with the rsEGFP2-GluA3 component in blue and the DL488-GluA2 component in magenta.