Figure 5. Calculated relative concentration error for distinguishing photoswitching from non-switching FPs in SV, with and without blinking.
The calculations are based on a decaying signal contribution of the psFP with different rate constants, assuming standard sedimentation conditions (12 mm solution column, 20°C, 50,000 rpm, similar to data shown in Figure 2c) and an s-value of 2.8 S. Shown are the predictions of Equation 6 for constant scanning (black) and blinking (magenta) with 2 min reset events occurring at 26 min and 69 min.