Table 1.

Experimentally derived model parameters describing the action of menthol and AITC on TRPM8 gating.

DOI: http://dx.doi.org/10.7554/eLife.17240.012

Parameter	Value	Source
z	0.82	(Voets et al., 2007)
${\displaystyle \mathrm{\Delta }\mathrm{\Delta }{G}_{AITC}}$	−2.7 ± 0.4 kJ mol−1	Steady-state activation curves (n = 7)
${\displaystyle \mathrm{\Delta }\mathrm{\Delta }{G}_{menthol}}$	−4.5 ± 0.4 kJ mol−1	Steady-state activation curves (n = 6)
Kd,AITC	2.9 ± 0.6 mM	Steady-state activation curves (n = 7)
Kd,menthol	21 ± 4 μM	Steady-state activation curves (n = 6)
${\displaystyle {\alpha }_0\left(0\right)}$	10.4 ± 1.2 s−1	Global kinetic fit (n = 14)
${\displaystyle {\beta }_0\left(0\right)}$	1.11 ± 0.15 ×103 s−1	Global kinetic fit (n = 14)
kon,AITC	95 ± 35 ×103 M−1 s−1	Global kinetic fit (n = 7)
kon,menthol	551 ± 210 ×103 M−1 s−1	Global kinetic fit (n = 7)

Displayed are values for the different parameters that determine the MWC model. For the global kinetic fits, cells were included for which current traces were fit at minimally tree ligand concentrations and two voltages. More details are provided in the text.