Table 1.
Primer sequences of immune-related genes used for quantitative RT-PCR analysis in this study
| Gene Symbol | Gene Name | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|---|
| CASP1 | Caspase 1 | CCTCAAGTTTTGCCCTTTAGAAAT | TGAAAGTCTGTGCTGCAGATAATG |
| CASP3 | Caspase 3 | AGGCCGACTTCCTGTATGCTT | TGACCCGTCCCTTGAATTTC |
| CCL2 | Chemokine (C-C motif) ligand 2 | CCCAAAGAAGCTGTAGTATTTG | TCTGGACCCATTCCTTATTG |
| CCL3 | Chemokine (C-C motif) ligand 3 | TGAGACCAGCAGCCTTTGCT | CAGATCTGCCGGTTTCTCTTG |
| CCL5 | Chemokine (C-C motif) ligand 5 | GTCGTCTTTGTCACTCGAAGGA | GATGTATTCTTGAACCCACTTCTTCTC |
| CCL11 | Chemokine (C-C motif) ligand 11 | TCACGGCCACTTCCTTCAC | GGGATCTTCTTACTGGTCATGGTAA |
| CCR1 | Chemokine (C-C motif) receptor 1 | GGGACCTTGAACCTTGAATTCATA | TGAGTCTGTGTTTCCAGAGTACTTTGT |
| CCR2 | Chemokine (C-C motif) receptor 2 | GGTCTGCTACGGGCTCACA | CCCGGCGTTTCTGTCTCAT |
| CCR3 | Chemokine (C-C motif) receptor 3 | CAGTCCTCGCTATCCA | TTTAGCTCTTCCTCTCCTCAT |
| CCR5 | Chemokine (C-C motif) receptor 5 | TCATAAAACCAGTGTGAAACAAATTG | TCATGTTACCCACAAAACCAAAGA |
| CX3CL1 | Chemokine (C-X3-C motif) ligand 1 | CTGGCCGCGTTCTTTCAT | TGGCACGTGATGTTGCATTT |
| CX3CR1 | Chemokine (C-X3-C motif) receptor 1 | GGGTGAGTGGCTGGCACTT | GATCCAATTCCGGGAAGGA |
| CXCL10 | Chemokine (C-X-C motif) ligand 10 | GGCCATAGGAAAACTTGAAATCA | CTCTTCTCATTGTTCTTTTTCATTGTG |
| IFNα1 | Interferon-α1 | CCTGGTGGTGGTGAGCTACTG | GCTCTCTTGTTCCTGAGGTTATGAG |
| IFNαR1 | Interferon (α, β and ω) receptor 1 | CAGTGCACACATCGCTTCAGA | ATTTTATTTGCCACTGTGGTGTTTAT |
| IFNβ1 | Interferon β1, fibroblast | GCGTTCCTGCTGTGCTTCTC | TGCTAGTGCTTTGTCGGAACTG |
| IFNγ | Interferon γ | CATTCATGAGCATCGCCAAGT | TCTGATGAGTTCATTGACAGCTTTG |
| IFNγR1 | Interferon γ receptor 1 | CCCCTTTCTCCATGATGACAGA | GCCGGGACAACTATTGTAAGGA |
| IL1α | Interleukin 1α | GCCATAGCCCATGATTTAGAAGA | TGCTTGACGATCCTTATCAATTTG |
| IL1β | Interleukin 1β | AACAGGCTCATCTGGGATCCT | TCAGGGACAGTTGCCATAGCT |
| IL1R1 | Interleukin 1 receptor, type I | AAGTGGAATGGGTCGGAAATT | TGAAGGGTGTTCCAAAAACTGA |
| IL1R2 | Interleukin 1 receptor, type II | TGCACACAGGAAGGGTTTCTG | AACCAGCAATCGTCTCCAAGA |
| IL2 | Interleukin 2 | TTCATTTTCCAGGCACTGAAGA | CCATGATGCTCACGTTTAAATTT |
| IL4 | Interleukin 4 | CAGACGTCCTTACGGCAACA | TGCGAAGCACCCTGGAA |
| IL5 | Interleukin 5 | CCATGAGCACAGTGGTGAAAGA | CGTCTCATTGCTCGTCAACAG |
| IL6 | Interleukin 6 | GCCCTTCAGGAACAGCTATGA | TGTCAACAACATCAGTCCCAAGA |
| IL6R | Interleukin 6 receptor | GCCCTTGCTGGTGGATGTT | GAGAGGGAGTGCTGCTTGGA |
| IL8Rα | Interleukin 8 receptor, α | GGTCTGCTACGGGCTCACA | CCCGGCGTTTCTGTCTCAT |
| IL10 | Interleukin 10 | CCCTGGGAGAGAAGCTGAAGA | CACTGCCTTGCTTTTATTCTCACA |
| IL10Rα | Interleukin 10 receptor, α | GCGCACACGGGACAGAAC | GCTGGAAAAATCTGGCTTCAA |
| IL11 | Interleukin 11 | CTGGATAGCGCTGTCCTCTTG | TGTCTCTCATCTGTGCAGCTAGTTG |
| IL12α | Interleukin 12α | TCTGGACCTGCCAAGTGTCTT | TGGCCGTCCTCACCATGT |
| IL12β | Interleukin 12β | AGAGGAGGAGTGTAACCAGAAAGGT | CGCCCCTTTGCATTGG |
| IL13 | Interleukin 13 | GCAACATCACACAAGACCAGAAG | TGTCAGGTCCACGCTCCAT |
| IL15 | Interleukin 15 | CCTTCTCAACAGTCACTTCTT | ACAGAGGCCAACTGGATA |
| IL16 | Interleukin 16 | CCAGTTCCCATCATCGTTAGC | CCAAACTTGACGCCTTCCA |
| IL17β | Interleukin 17β | AGCTGCAGGCTGACTTAATGG | AGATGGAGATGGCAAGAAGGAA |
| IL18 | Interleukin 18 | AAACCCGCCTGTGTTCGA | TCAGTCTGGTCTGGGATTCGT |
| IL33 | Interleukin 33 | CCAAGGAACTTCACTGCTAACAGA | GTAACATCCATTCTCCAAAACAAAAC |
| IRF1 | Interferon regulatory factor 1 | CATTCCCGGGTACCCTCTGT | TCTCTAGCCAGGGTCTCATTCG |
| IRF2 | Interferon regulatory factor 2 | AAGGAAACAACGCCTTCAGAGT | TTCTCTGTCTTTGGTTTCTTTCCTTT |
| IRF3 | Interferon regulatory factor 3 | CTGGCTCAGGAACTGCACTGT | CCCAGGTCCAGCTGAGACA |
| IRF4 | Interferon regulatory factor 4 | CAGACTGCCGGCTGCATAT | CCAGGTTGCTAACATCATAGGTATGT |
| IRF6 | Interferon regulatory factor 6 | CATAGAGATTCCAAACGCTTCCA | CAAGCCTTAAAAATGGTGTTTTCTT |
| IRF7 | Interferon regulatory factor 7 | GCTCTGGAGAACAGGGAAGAAG | GTTTGCAACCCAGCATTTCCT |
| IRF8 | Interferon regulatory factor 8 | TGGTGACTGGGTATACTGCCTATG | TGCCCCCGTAGTAAAAGTTGA |
| IRF9 | Interferon regulatory factor 9 | GGAAGGAGCTGGCACAACTG | AGGAAGCGGATTCTCCATGA |
| TGFβ1 | Transforming growth factor, β1 | AGAAGTCACCCGCGTGCTA | TGTGTGATGTCTTTGGTTTTGTCA |
| TLR4 | Toll-like receptor 4 | CCCTCATGACATCCCTTATTC | CAATTTCTCACAACTTCAGTGG |
| TNF | Tumor necrosis factor (TNF superfamily, member 2) | CCTCAGCCTCTTCTCATT | GGAACTTCTCCTCCTTGT |
| TNFRSF1α | Tumor necrosis factor receptor superfamily, member 1α | TGCCCATCCAAAGAATAATTCC | TGGGCTTGGACAGTCACTCA |
| TNFRSF1β | Tumor necrosis factor receptor superfamily, member 1β | GCCTAGCCAAACTCCACACATC | TCAGTCCAACGATCAGACCAAT |