Table 2.

Genetic variances included in GRS models

	SNP	Gene	Minor allele	Reported effect allele	Risk allele/weight loss lowering allele	MSE (%)
GRSwBMI, weighted BMI	rs571312	MC4R	A	A	C	23.16
	rs3810291	TMEM160	G	A	G	19.65
	rs1555543	PTBP2	A	C	A	18.58
	rs206936	NUDT3	G	G	G	17.58
	rs987237	TFAP2B	G	G	G	17.49
	rs4836133	ZNF608	A	A	A	17.12
	rs2241423	MAP2K5	A	G	G	13.10
GRSwWHR, weighted WHR	rs1443512	HOXC13	A	A	A	13.15
	rs4846567	LYPLAL1	A	G	G	11.24
	rs1011731	DNM3-PIGC	G	G	A	10.32

The listed single nucleotide polymorphisms (SNP) induced a mean squared error (MSE) above 10 % in the random forest model. These variants were subsequently included in the respective GRS model (weighted BMI model (GRS_wBMI) and weighted waist-hip ratio model (GRS_wWHR)). Those alleles were defined as risk alleles that contributed least to the weight loss following Roux-en Y gastric bypass surgery. Interestingly, the A allele of the TMEM160-related SNP rs3810291 was reported to be associated with higher BMI by Speliotes et al. In our study, the allele, G, is associated with EBMIL