Figure 6.
StemID Predicts Human Pancreatic Pluripotent Cells
(A) t-SNE map of transcriptome similarities of human pancreatic cells. RaceID2 clusters are highlighted with different numbers and colors. Cell types identified based on marker gene expression are shown. For ductal cells, marker genes of sub-populations are shown.
(B) Heatmap showing the average expression of known cell type markers across all clusters with more than five cells. For each gene, the sum of expression values over all clusters is normalized to one.
(C) Transcript counts (color legend) of the ductal sub-type markers CEACAM6, FTH1, KRT19, and SPP1 are highlighted in the t-SNE map.
(D) Inferred pancreatic lineage tree. Only significant links are shown (p < 0.01). The color of the link indicates the −log10 p value. The color of the vertices indicates the entropy. The thickness indicates the link score reflecting how densely a link is covered with cells (Experimental Procedures).
(E) Barplot of StemID scores for pancreatic clusters.
(F) Pseudo-temporal expression profiles for INS and FTH1. The transcript count is plotted for cells on the link, connecting clusters 4, 8, and 6. Cells are ordered by the projection coordinate.
In (B), (D), and (E), only clusters with more than five cells were analyzed. See also Figure S5.