Figure 4.

Changes in CF and LVDP in isolated perfused rat hearts after infusion of serotonin‐supplemented peripheral plasma from healthy volunteers. CF, coronary flow; LVDP, left ventricular developed pressure. Supplemented plasma with 0.5 μmol/L is equivalent to coronary arterial plasma with its mean serotonin concentration, 1.0 μmol/L and 3.0 μmol/L represent approximately the range of serotonin concentrations measured in aspirate plasma and 6.0 μmol/L represents the serotonin concentration corrected by dilution in the perfusate. Statistical comparison by one‐way repeated measures ANOVA with Bonferroni's correction. (A) Representative original registrations of changes in CF and LVDP in isolated perfused rat hearts. Plasma infusion (2 mL at a rate of 2 mL/min, plasma diluted by a factor of 6.4) into hearts without and with pharmacological NOS inhibition by L‐NAME. Each original registration approximates the mean min/max value of the group after serotonin‐supplemented plasma infusion. Gray box: time frame in which min and max values were extracted to calculate changes in CF and LVDP, expressed as Δ mean baseline, respectively. (B) Mean of min and max values after plasma infusion are presented as absolute values after infusion and calculated as Δ mean baseline.