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. 2016 Aug 1;4(15):e12876. doi: 10.14814/phy2.12876

Figure 1.

Figure 1

(A) Basal and insulin‐stimulated glucose uptakes in rat soleus muscle in contralateral non‐immobilized and immobilized limbs at the end of 6‐h hindlimb immobilization. Muscles were dissected out at the end of 6‐h unilateral hindlimb immobilization. All muscles were incubated in glucose‐free medium in the absence or presence (50 or 10,000 μU/mL) of insulin for 20 min, followed by the measurement of 2‐deoxyglucose uptake. Values are means ± SE (n = 5–6). *P < 0.05 versus contralateral non‐immobilized limbs with same insulin concentration. † P < 0.05 versus 0 μU/mL insulin. (B) Total GLUT4 protein concentration at the end of 6‐h hindlimb immobilization. Muscles were dissected out and frozen at the end of 6‐h unilateral hindlimb immobilization. Muscle lysates were separated with SDS‐PAGE, and blots were analyzed for GLUT4 protein content. Values are means ± SE (n = 8).