Figure 3.

Activation of MAPK pathways in contralateral non‐immobilized and immobilized limbs at the end of 6‐h hindlimb immobilization. Muscles were dissected out and frozen at the end of 6‐h unilateral hindlimb immobilization. Muscle lysates were separated with SDS‐PAGE, and blots were analyzed for phosphorylated JNK (A), phosphorylated p38 MAPK (B), and phosphorylated ERK (C). Blots were then stripped and analyzed for total abundance of each protein. Values are means ± SE (n = 7–8). *P < 0.05 versus the contralateral non‐immobilized limbs.