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. 2016 Aug 10;20(2):250–258. doi: 10.1016/j.chom.2016.07.007

Figure 1.

Figure 1

Direct Ex Vivo Phagolysosomal Localization of M. tuberculosis

Images of red fluorescent Mtb H37Rv from infected lung. Cells were sorted from lung tissue at 13 days post-infection (dpi) (A) or 19 dpi (B) and stained with LysoTracker (LT) Green dye.

(A) The top images show an infected macrophage in which the Mtb colocalizes with LT, while the bottom images show an infected macrophage in which the Mtb does not colocalize with LT. Representative of two mice. Scale bar, 5 μm.

(B) Arrowheads depict bacteria that colocalize with LT, while arrows depict bacteria that do not colocalize with dye. The dotted arrow marks partially colocalized bacteria. Representative of three mice. Scale bar, 25 μm.