Figure 4.
Modeling the Inflammatory Response to Competing Attractant Cues
(A–F) For wounds close together, attractant gradients overlap and mimic a single, very large wound (A and B), predicting less biased migration in the inter-wound region. For wounds far apart, attractant gradients will not interact (E and F), and hemocytes respond as for two single wounds. For wounds of intermediate distance apart (C), attractant gradients will strongly overlap by 25 min (D), creating shallower gradients in the inter-wound region.
(G–O) In vivo imaging (G–I) with representative hemocyte tracks; srp-Gal4 drives UAS-nuclear-red-stinger. Two close wounds caused reduced bias in the inter-wound region (red and blue, J) while two wounds far apart behaved separately, with slightly reduced bias on the outer sides of the wing (L). For wounds at an intermediate distance, cell bias was significantly lower in the inter-wound region (K and N) for all time points examined (O), with clear hemocyte confusion in vivo (boxed cell tracks in H and plotted trajectories in M). Gray boxes indicate wound position (J–L).