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. 2016 Aug 2;2016:4196572. doi: 10.1155/2016/4196572

Figure 3.

Figure 3

Role of RhoA/ROCK signaling in HIV-1 Tat-induced changes in RAGE. Exposure to HIV-1 Tat resulted in markedly higher levels of RAGE protein (a) and mRNA (b) and stronger immunoreactivity (c) compared with the untreated group. hCMEC/D3 cells were pretreated with HF (30 μmol/L) 2 h prior to HIV-1 Tat treatment for 24 h. RAGE protein and mRNA levels and immunoreactivity were significantly decreased with coexposure to HF and HIV-1 Tat compared with HIV-1 Tat only. Data are expressed as means ± standard error of the mean (n = 3, values determined by the ratio to GAPDH, for (a), n = 5 for (b)). ∗ p < 0.05 and ∗∗ p < 0.01 versus control; # p < 0.05 versus HIV-1 Tat.