Table 4.
Tabulation of all proteins identified in common between two biological replicates of eight-plex iTRAQ-labeled β2*-nAChRs immunoisolated with mAb295 from mouse cortex, indicating which proteins have been identified in human temporal cortex or whole mouse brain (Iso, isoform of protein identified; GN, gene name)
| Accession_MOUSE | Description | Human Ctx | Mouse brain |
|---|---|---|---|
| 1433T | 14-3-3 protein theta GN = Ywhaq | Iso | Iso |
| ACHA4 | Neuronal acetylcholine receptor subunit α4 GN = Chrna4 | Yes | Yes |
| ACHA5 | Neuronal acetylcholine receptor subunit α5 GN = Chrna5 | Iso | Iso |
| ACHB2 | Neuronal acetylcholine receptor subunit β2 GN = Chrnb2 | Yes | Yes |
| ACPM | Acyl carrier protein, mito GN = Ndufab1 | No | Yes |
| ACTG; ACTB | Actin, cytoplasmic 2 GN = Actg1; actin, cytoplasmic 1 GN = Actb | Iso | Yes |
| ADT1 | ADP/ATP translocase 1 GN = Slc25a4 SV = 4 | Yes | Yes |
| ADT2 | ADP/ATP translocase 2 GN = Slc25a5 | Yes | Yes |
| AINX | α-Internexin GN = Ina | No | Yes |
| AT1A3 | Sodium/potassium-transporting ATPase subunit α3 GN = Atp1a3 | Yes | Yes |
| AT1B1 | Sodium/potassium-transporting ATPase subunit β1 GN = Atp1b1 | Yes | Yes |
| AT5F1 | ATP synthase subunit b, mito GN = Atp5f1 | Iso | Yes |
| ATP5H | ATP synthase subunit d, mito GN = Atp5h | Iso | Yes |
| ATP5I | ATP synthase subunit e, mito GN = Atp5i | Iso | Yes |
| ATPA | ATP synthase subunit α, mito GN = Atp5a1 | Yes | Yes |
| ATPB | ATP synthase subunit β, mito GN = Atp5b | Yes | Yes |
| ATPD | ATP synthase subunit delta, mito GN = Atp5d | Iso | Yes |
| C1QA | Complement C1q subcomponent subunit A GN = C1qa | No | No |
| CALM | Calmodulin GN = Calm1 | Yes | Yes |
| CLH1 | Clathrin heavy chain 1 GN = Cltc | Yes | No |
| CMC1 | Calcium-binding mito carrier protein Aralar1 GN = Slc25a12 | No | Yes |
| CN37 | 2',3'-cyclic-nucleotide 3'-phosphodiesterase GN = Cnp | No | Yes |
| EAA2 | Excitatory amino acid transporter 2 GN = Slc1a2 | Yes | Yes |
| G3P | Glyceraldehyde-3-phosphate dehydrogenase GN = Gapdh | Yes | Yes |
| GBB1 | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit β1 GN = Gnb1 | Yes | Yes |
| GFAP | Glial fibrillary acidic protein GN = Gfap SV = 4 | Yes | Yes |
| GLNA | Glutamine synthetase GN = Glul SV = 6 | No | Yes |
| GNAO | Guanine nucleotide-binding protein G(o) subunit α GN = Gnao1 | Yes | Yes |
| KCC2A | Calcium/calmodulin-dependent protein kinase type II subunit α GN = CaMKIIa | Yes | Yes |
| KCC2B | Calcium/calmodulin-dependent protein kinase type II subunit β GN = CaMKIIb | Yes | Yes |
| M2OM | Mito 2-oxoglutarate/malate carrier protein GN = Slc25a11 | No | Yes |
| MBP | Myelin basic protein GN = Mbp | Yes | Yes |
| MPCP | Phosphate carrier protein, mito GN = Slc25a3 | Yes | Yes |
| MYH10 | Myosin-10 GN = Myh10 | Yes | Yes |
| MYL6 | Myosin light polypeptide 6 GN = Myl6 | Yes | Yes |
| NDUA2 | NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 2 GN = Ndufa2 | Iso | Yes |
| NDUA4 | NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 4 GN = Ndufa4 | Yes | Iso |
| NDUA5 | NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 5 GN = Ndufa5 | Iso | Yes |
| NDUA6 | NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 6 GN = Ndufa6 | Iso | Yes |
| NDUA7 | NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 7 GN = Ndufa7 | Iso | Yes |
| NDUA9 | NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 9, mito GN = Ndufa9 | Iso | Yes |
| NDUAA | NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 10, mito GN = Ndufa10 | Iso | Yes |
| NDUAC | NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 12 GN = Ndufa12 | Iso | Yes |
| NDUAD | NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 13 GN = Ndufa13 | Iso | Yes |
| NDUB3 | NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 3 GN = Ndufb3 | Iso | Yes |
| NDUB4 | NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 4 GN = Ndufb4 | Iso | Yes |
| NDUB5 | NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 5, mito GN = Ndufb5 | Iso | Yes |
| NDUB6 | NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 6 GN = Ndufb6 | Iso | Yes |
| NDUB7 | NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 7 GN = Ndufb7 | Iso | Yes |
| NDUB8 | NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 8, mito GN = Ndufb8 | Iso | Yes |
| NDUB9 | NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 9 GN = Ndufb9 | Iso | Yes |
| NDUC2 | NADH dehydrogenase [ubiquinone] 1 subunit C2 GN = Ndufc2 | Iso | Yes |
| NDUS1 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mito GN = Ndufs1 | Iso | Yes |
| NDUS2 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mito GN = Ndufs2 | Iso | Yes |
| NDUS3 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mito GN = Ndufs3 | Yes | Yes |
| NDUS4 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mito GN = Ndufs4 | Iso | Yes |
| NDUS5 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 GN = Ndufs5 | Iso | Yes |
| NDUS6 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mito GN = Ndufs6 | Iso | Yes |
| NDUS7 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mito GN = Ndufs7 | Iso | Yes |
| NDUS8 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mito GN = Ndufs8 | Iso | Yes |
| NDUV1 | NADH dehydrogenase [ubiquinone] flavoprotein 1, mito GN = Ndufv1 | Iso | Yes |
| NDUV2 | NADH dehydrogenase [ubiquinone] flavoprotein 2, mito GN = Ndufv2 | Yes | Yes |
| NEST | Nestin GN = Nes | No | No |
| NFH | Neurofilament heavy polypeptide GN = Nefh | Iso | Iso |
| NFL | Neurofilament light polypeptide GN = Nefl SV = 5 | Yes | Yes |
| NFM | Neurofilament medium polypeptide GN = Nefm SV = 4 | Yes | Yes |
| OCAD2 | OCIA domain-containing protein 2 GN = Ociad2 | No | Yes |
| ODO2 | Dihydrolipoyl-lysine residue succinyltransferas 2-oxoglutarate dehydrogenase complex, mito GN = Dlst | Yes | Yes |
| ODPA | Pyruvate dehydrogenase E1 component subunit α, somatic form, mito GN = Pdha1 | No | Yes |
| ODPB | Pyruvate dehydrogenase E1 component subunit β, mito GN = Pdhb | No | Yes |
| PHB | Prohibitin GN = Phb | No | Yes |
| PHB2 | Prohibitin-2 GN = Phb2 | No | Yes |
| RAB3A | Ras-related protein Rab-3A GN = Rab3a | Iso | Yes |
| SFXN3 | Sideroflexin-3 GN = Sfxn3 | No | Yes |
| SNP25 | Synaptosomal-associated protein 25 GN = Snap25 | Yes | Yes |
| SPTB2 | Spectrin β chain, nonerythrocytic 1 GN = Sptbn1 | Yes | Yes |
| SPTN1 | Spectrin α chain, nonerythrocytic 1 GN = Sptan1 SV = 4 | Yes | Yes |
| STX1B | Syntaxin-1B GN = Stx1b | Yes | Yes |
| SYPH | Synaptophysin GN = Syp | No | No |
| SYT1 | Synaptotagmin-1 GN = Syt1 | Yes | Yes |
| TBA4A | Tubulin α4A chain GN = Tuba4a | Yes | Yes |
| TBB2A | Tubulin β2A chain GN = Tubb2a | Yes | Yes |
| TBB3 | Tubulin β3 chain GN = Tubb3 | Yes | Yes |
| TBB4A | Tubulin β4A chain GN = Tubb4a | Yes | Yes |
| TBB4B | Tubulin β4B chain GN = Tubb4b | Yes | Yes |
| TBB5 | Tubulin β5 chain GN = Tubb5 | Yes | Yes |
| THY1 | Thy-1 membrane glycoprotein GN = Thy1 | Yes | No |
| TPM3 | Tropomyosin α3 chain GN = Tpm3 | Iso | No |
| VA0D1 | V-type proton ATPase subunit d 1 GN = Atp6v0d1 | Yes | Iso |
| VATA | V-type proton ATPase catalytic subunit A GN = Atp6v1a | Yes | Yes |
| VDAC1 | Voltage-dependent anion-selective channel protein 1 GN = Vdac1 | No | Yes |
| VDAC2 | Voltage-dependent anion-selective channel protein 2 GN = Vdac2 | No | Yes |
| VIME | Vimentin GN = Vim | No | Yes |
| VPP1 | V-type proton ATPase 116 kDa subunit a isoform 1 GN = Atp6v0a1 | Yes | Iso |