Figure 7. The effect of ribonuclease (RNase) 5 on G₁ to S cell cycle progression via PI3-kinase/Akt activation in cultured human corneal endothelial cells (CECs).

(A–D) Representative immunoblotting and quantitative analysis of band density of phosphorylated p27Kip1 (p-p27Kip1, A), cyclin (Cyc) D1 (B), cyc D3 (C) and cyc E (D) in cultured human CECs with or without RNase 5 (5 μg/mL, 24 hours), and in the presence or absence of LY294002 (20 μM) or neomycin (1 mM) co-treatment. P27Kip1 (A) **p = 0.005, vs. control; ^##p = 1.86e-4, RNase 5 treatment with vs. without LY294002; ^##p = 0.007, RNase 5 treatment with or without neomycin. Cyc D1 (B) *p = 0.046, vs. control; ^#p = 0.042, RNase 5 treatment with vs. without LY294002. Cyc D3 (C) *p = 0.042, vs. control; ^##p = 0.001, RNase 5 treatment with vs. without LY294002; ^##p = 0.003, RNase 5 treatment with vs. without neomycin. Cyc E (D) **p = 3.63e-4, vs. control; ^##p = 3.45e-4, RNase 5 treatment with vs. without LY294002; ^##p = 2.53e-4, RNase 5 treatment with or without neomycin. Statistical analysis was performed with ANOVA followed by Bonferroni’s post-hoc analysis. n = 4 independent experiments. β-actin was used as a loading control for Western blotting, and the small gaps indicate skipped lanes from the same membrane. Values represent the mean ± s.e.m. Full-length gels are presented in Supplementary Fig. S7.