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. 2016 Aug 16;6:31222. doi: 10.1038/srep31222

Figure 1. Insulin induces INSR exon 11 inclusion in pancreatic beta cells.

Figure 1

(A) MIN6 cells were starved for 24 hours in 0.1% serum and then stimulated for 24 hours with 100 nM insulin. Total RNA was extracted and subjected to RT-PCR using primers from exon 10 and 12 of INSR. Actin was used as control. (B) Human Islets were stimulated with insulin and subjected to RT-PCR as in (A). GAPDH served as an RT-PCR control. (C) Mouse Islets were stimulated with insulin and subjected to RT-PCR as in (A). GAPDH served as an RT-PCR control. The left panels are representative gels and the right panels are quantification of % exon 11 inclusion from 3 individual experiments. Error bars, SD; n = 3, *p < 0.05.