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. 2016 Aug 16;6:31222. doi: 10.1038/srep31222

Figure 3. GCK1 activation upregulates SRSF1 and induces INSR exon 11 inclusion.

Figure 3

(A) MIN6 cells were exposed to DMEM medium without glucose containing DMSO with or without 25 μM GKA50 for 48 hours. Total RNA was extracted and subjected to RT-PCR using primers from exon 10 and 12 of INSR. Tubulin was used as control. (B) Total SRSF1 mRNA levels from cells described in (A) were detected by qRT-PCR using primers from exon 2 and 3.Tubulin was used for normalization. (C) Western blot of lysates from cells described in (A). (D) Total RNA was isolated from mouse islets of control mice (n = 5) or mice overexpressing GCK1 specifically in β-cells (n = 5) and subjected to RT-PCR using primers from exon 10 and 12 of INSR. GAPDH served as an RT-PCR control. (E) Graph showing quantitation of % exon 11 inclusion in (D). (F) Q-RT-PCR of insulin expression in samples described in (D).