Figure 4. SRSF1 knockdown blocks INSR exon 11 inclusion upon insulin stimulation.

(A) MIN6 cells were transfected with the indicated siRNAs for 96 hours. RNA was extracted and SRSF1 levels were detected by Q-RT-PCR. (B) Cells described in (A) were lysed and SRSF1 protein levels were detected by Westen blot. β-catenin was used as loading control. (C) INSR exon 11 inclusion was detected in RNA from cells described in (A) by Q-RT-PCR. (D) MIN6 cells were transfected with the indicated siRNAs for 36 hours, starved in low serum over night, and induced with insulin for 24 hours. INSR exon 11 inclusion was detected by Q-RT-PCR. Error bars show SD; n = 3.