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. 2016 Aug 16;6:31222. doi: 10.1038/srep31222

Figure 5. High glucose and lipid levels induce INSR exon 11 skipping in MIN6 cells.

Figure 5

(A,B) MIN6 cells were incubated in glucose-free DMEM medium. After 24 hours medium was replaced with DMEM medium without or with 12.5 mM or 25 mM glucose for 24 and 48 hours. After stipulated period of time, RNA was isolated and subjected to RT-PCR to detect the splicing of INSR. Tubulin served as a RT-PCR control. (C) MIN6 cells were incubated in normal MIN6 medium with 0.5% (w/v) BSA with or without 0.5 mM palmitate for 48 hours. After stimulation, RNA was isolated and subjected to RT-PCR to detect the splicing of INSR. Tubulin served as a RT-PCR control. The right panels represent quantification of % exon 11 inclusion from 3 individual experiments. Error bars, SD; n = 3, *p < 0.05.