Figure 7. Induction of INSR exon 11 skipping sensitized MIN6 cells to lipotoxicity-induced apoptosis.

(A) MIN6 cells were transfected with 2.5 μM INSR specific or scrambled RNA oligos. 24 h after transfection medium was replaced with medium containing 0.1% serum with 0.5% (w/v) BSA with or without 0.5 mM palmitate for 36 or 48 hrs. Total RNA was extracted and subjected to RT-PCR using primers from exon 10 to 12 of INSR. (B) Q-RT-PCR of cells described in (A). (C,D) Left panels. Cell death in cells described in (A) was measured by trypan blue exclusion assay. Right panels: Western blot of the cells described in (A) probed with antibodies to cleaved Caspase -3. β-catenin was used as a loading control.