Figure 8. Induction of INSR exon 11 skipping inhibits insulin–induced activation of the Ras-MAPK-ERK pathway.

(A) (Left): Western blot of MIN6 cells starved for 24 hours in 0.1% serum and then stimulated with 100 nM insulin for 1 hour. β-catenin was used as loading control. (Right): Western blot of MIN6 cells transfected with either 2.5 μM INSR specific or scrambled RNA oligos, serum starved, and then stimulated for 1 hour with insulin. β-catenin was used as loading control. (B) Schematic representation of the regulation of INSR splicing by insulin signaling through the MAPK-ERK pathway.