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. 2016 Aug 16;6:31562. doi: 10.1038/srep31562

Figure 7. Detection of Th2 and Treg cells and cytokines after drug intervention.

Figure 7

The combined use of glucocorticoid and IL-2 had no obvious impact on the whole body. Female BALB/c mice were immunized with OVA i.p on days 1 and 8, followed by intranasal (i.n) 2% OVA challenges on days 9–14. 50,000 IU IL-2 plus 10 μg budesonide (Bud) were administrated intratracheally on days 12–14. On day 15, mice were sacrificed and analyzed by flow Tcytometry or ELISA. (a–c) Detection of CD4+FoxP3+ Treg cells, CD4+T-bet+ Th1 cells and CD4+GATA3+ Th2 cells in spleens after 3 days use of 50,000 IU IL-2(PEG) combined with 10 μg budesonide (Bud) by flow cytometry. (d) Measurement of cytokines by ELISA in serum after 3 days use of 50,000 IU IL-2(PEG) combined with 10 μg Bud. Data are presented as means ± SEM (n ≥ 6 per group and data point) from 2 independent experiments. p > 0.5. Treated group versus untreated group by Student’s t test.