Figure 4. Stability of plasmid pMTLts in C. ljungdahlii without selective pressure under permissive (30 °C) and non-permissive (37 °C) temperatures.

For C. ljungdahlii (pMTLts) thiamphenicol resistant cells were counted after plating 10⁻⁴ and 10⁻⁵ dilutions of the non-selectively grown cultures. For comparison of plating efficiencies C. ljungdahlii wildtype was plated in the same dilutions without thiamphenicol. WT (wildtype): n = 1; pMTLts (cells carrying plasmid pMTLts): n = 3. CFU = colony forming units.