Table 3. Transformation efficiencies for different clostridial species with plasmid pMTL82151 (catP, pBP1) or pGlow-CKXNPp1, pGlow- CKXNBs2 (ermB, pIM13).
Organism | Plasmid | Et a(transformants/μg DNA), mean ± SD (n) |
---|---|---|
C. acetobutylicum | pMTL82151 | (4.61 ± 4.3) × 102 (6) |
pGlow-CKXNPp1 | (9.03 ± 3.38) × 102 (3) | |
pGlow-CKXNBs2 | (9.09 ± 5.07) × 102 (3) | |
C. pasteurianum | pMTL82151 | (4.03 ± 3.3) × 102 (6) |
C. perfringens | pMTL82151 | (4.61 ± 1.08) × 103 (6) |
aEt = transformation efficiency.