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. 2016 Aug 16;6:31518. doi: 10.1038/srep31518

Table 4. Bacterial strains and plasmids used in this work.

Strain or plasmid Relevant characteristics Source
strains
 Clostridium ljungdahlii DSM 13528 wildtype DSMZ
 C. ljungdahlii (pGlow-CKXNPp1) carrying plasmid pGlow-CKXNPp1 this work
 C. ljungdahlii (pGlow-CKXNBs2) carrying plasmid pGlow-CKXNBs2 this work
 C. ljungdahlii (pMTLts) carrying plasmid pMTLts this work
 C. ljungdahlii (pMTLts-Bs2) carrying plasmid pMTLts-Bs2 this work
 Clostridium acetobutylicum DSM 792 wildtype DSMZ
 C. acetobutylicum (pGlow-CKXNPp1) carrying plasmid pGlow-CKXNPp1 this work
 C. acetobutylicum (pGlow-CKXNBs2) carrying plasmid pGlow-CKXNBs2 this work
 Clostridium perfringens DSM 756 wildtype DSMZ
 Clostridium pasteurianum DSM 525 wildtype DSMZ
 E. coli DH5αMCR F- endAI supE44 thi-J A- recAl gyrA96 relAI deoR A(lacZYA-argF)U169 08OdlacZAM15 mcrA A(mrr hsdRMS mcrBC) 44
plasmids
 pGlow-CKXNPp1 Gram+: pIM13, ermB; Gram−: ColE1, AmpR; application: evoglow-Pp1-Cl Evocatal, Germany
 pGlow-CKXNBs2 Gram+: pIM13, ermB; Gram−: ColE1, AmpR; application: evoglow-Bs2-Cl Evocatal, Germany
 pMTL82151 Gram+: pBP1, catP; Gram−: ColE1, catP; application: MCS 28
 pMTL83151 Gram+: pCB102; catP; Gram−: ColE1, catP; application: MCS 28
 pSS60 Gram+: ermB, pWV01ts; Gram−: ColE1, AmpR gift from Peter Dürre, Ulm, Germany
 pMTL85141 Gram+: pIM13, catP; Gram−: ColE1, catP; application: MCS 28
 pMTLts Gram+: pWV01ts, catP; Gram−: ColE1, catP; application: MCS this study
 pMTLts-Bs2 Gram+: pWV01ts, catP; Gram−: ColE1, catP; application: evoglow-Bs2-Cl this study
 pAN1 Gram−: p15A, catP; application: Φ3tI methyltransferase 32
 pJET_AmpR subcloned AmpR cassette from pUC18 this study
 pANA1 Gram−: p15A, AmpR; application: Φ3tI methyltransferase this study