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. 2016 Aug 16;6:31514. doi: 10.1038/srep31514

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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GdnHCl-induced equilibrium unfolding of DehI monitored by (A) Far-UV CD, (B) intrinsic fluorescence and (C) SAXS. Top panels: GdnHCl titration series of far-UV CD and fluorescence emission spectra of DehI recorded from 0 to 5.7 M GdnHCl with a linear gradient with equal spacing, colored ramped from purple to red. Kratky plots of the SAXS data collected in the presence of 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 5, and 6M GdnHCl. Middle panels: SVD analysis outputs of far-UV CD, intrinsic fluorescence and SAXS data. Insets: the percentage and auto-correlation coefficients individual components. Residuals of data fitting are shown below. Bottom panels: the population of native state (solid line), intermediate state (dashed line) and unfolded state (dotted line) for DehI as a function of GdnHCl concentration. Populations of individual states are calculated using the thermodynamic parameters obtained from the fitting to a three-state or four-state folding model.