Table 1. Thermodynamic parameters for the fit of equilibrium unfolding data of DehI and variants.

DehI	wt	(kcal mol−1)	mN–I(kcal mol−1 M−1)	(M)	(kcal mol–1)	mI–I’(kcal mol−1 M−1)	(M)	(kcal mol−1)	mI/I′–U(kcal mol−1 M−1)	(M)
SAXS		2.75 ± 1.10	3.18 ± 1.23	0.87 ± 0.08				9.27 ± 5.52	2.70 ± 1.60	3.44 ± 0.16
Far-UV CD	wt	3.71 ± 1.31	3.24 ± 1.11	1.15 ± 0.10	5.40 ± 2.91	2.40 ± 1.28	2.25 ± 0.16	7.02 ± 1.20	1.98 ± 0.33	3.55 ± 0.10
	W34	3.10 ± 0.64	8.27 ± 1.63	0.37 ± 0.02	4.38 ± 0.67	2.53 ± 0.34	1.31 ± 0.15	4.87 ± 0.57	1.31 ± 0.15	3.71 ± 0.06
	W53	5.57 ± 0.95	8.57 ± 1.43	0.65 ± 0.02	5.55 ± 0.05	3.00 ± 0.51	1.85 ± 0.09	5.68 ± 0.02	1.48 ± 0.24	3.85 ± 0.08
	W196	5.36 ± 0.03	7.21 ± 0.73	0.74 ± 0.05	6.10 ± 0.09	3.74 ± 0.90	1.63 ± 0.10	4.51 ± 0.01	1.28 ± 0.17	3.54 ± 0.07
Fluorescence	wt	6.04 ± 0.33	6.60 ± 0.36	0.91 ± 0.01	9.92 ± 0.58	5.30 ± 0.30	1.87 ± 0.03	7.40 ± 0.32	2.02 ± 0.09	3.66 ± 0.02
	W34	4.83 ± 0.54	9.71 ± 1.08	0.50 ± 0.01	1.69 ± 0.08	0.99 ± 0.16	1.69 ± 0.08	6.49 ± 0.38	1.75 ± 0.10	3.70 ± 0.04
	W53	4.91 ± 0.34	6.74 ± 0.46	0.73 ± 0.01	8.88 ± 0.66	5.58 ± 0.40	1.59 ± 0.03	6.31 ± 0.41	1.72 ± 0.11	3.68 ± 0.05
	W196	5.55 ± 0.60	6.54 ± 0.70	0.85 ± 0.01	2.27 ± 0.24	1.38 ± 0.14	1.64 ± 0.06	4.21 ± 0.37	1.24 ± 0.10	3.41 ± 0.13

All experiments were recorded under the same buffer condition.