Figure 5. Mutation of c-Kit increases osteoblast differentiation and osteoprogenitors.

(A) PCR analysis using primers to amplify product from either WT (left) or W^sh/W^sh (right) genomic DNA. (B) ALP and alizarin red staining in calvarial osteoblasts at day 7 and 21, respectively. (C) The number of ALP-positive colony-forming unit (CFU-ALP) and alizarin red-positive CFU (CFU-OB) was increased in W^sh/W^sh osteoblasts. The same tissue culture plates were stained with toluidine blue to determine CFU-fibroblasts (CFU-F). CFU-F number was not changed. Results are mean ± SEM. *p < 0.05 versus WT.