Figure 7. Mutation of c-Kit increases Wnt10b mRNA expression and protein level in osteoclasts.
(A) ALP staining of osteoblast cultures treated with conditioned medium derived from either Wsh/Wsh or WT osteoclasts (B) qPCR analysis of osteoclast mRNA expression. (C) Confocal immunofluorescence images of osteoclasts generated on dentin slices and immunolabeled for nuclei (TO-PRO3, blue), Wnt10b (green) and actin (rhodamine phalloidin, red). (D) Western blot analysis of conditioned medium from WT and Wsh/Wsh osteoclast was performed with an anti-Wnt10b antibody (left). The same membrane was stained with Ponceau S (right) as a loading control. Results are mean ± SEM. *p < 0.05 versus WT.