Figure 8. Blocking Wnt10b attenuates ALP activity and mineralization induced by W^sh/W^sh osteoclast-conditioned medium.

(A) Calvarial osteoblasts were treated with either WT or W^sh/W^shosteoclast-conditioned medium with or without DKK1. ALP activity (n = 5 per group) and mineralization (n = 5 per group) were quantified. (B) Osteoblasts were cultured with either WT or W^sh/W^shosteoclast-conditioned medium pretreated with either isotype control (Ctrl) or Wnt10b antibody (Wnt10b). ALP activity (n = 5 per group) and mineralization (n = 5 per group) were quantified. Results are mean ± SEM. *p < 0.05 versus WT and ⁺ p < 0.05 versus corresponding controls.