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. 2016 Aug 16;6:31132. doi: 10.1038/srep31132

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Copyright © 2016, The Author(s)

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(A) Scan chart of AFP-L3 standard substances detected by the AFP/LCA-coated chips. AFP antibodies and LCA were applied to the slides. a: AFP antibody, 0.5 mg/ml; b: LCA, 4 mg/ml. Serum samples with different concentrations of AFP-L3 were used: (1–5) 100, 50, 25, 12.5, and 6.25 ng/ml; (6–9) 100, 50, 25, and 12.5 ng/ml; (10) blank control. (B) The standard curve chart and the regression equation of AFP-L3 determined using the AFP/LCA protein microarray assay.