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. 2016 Aug 16;6:31607. doi: 10.1038/srep31607

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Apoptosis cells were detected using the Annexin V-FITC/PI double staining after exposure to by241 at different concentrations (0, 2.5, 5, 10 μM) and analyzed by flow cytometry and the apoptotic cell rate was analyzed after Annexin V-FITC/PI staining. (A,B) MGC-803 were treated for 12 h or 24 h; (E,F) EC9706 were treated for 12 h or 24 h; (C,D) Statistical analysis of apoptotic cells of (A,B); (G,H) Statistical analysis of apoptotic cells of (E,F); data are presented as the mean ± SD of three independent experiments. *P < 0.05 and **P < 0.01 were considered statistically compared to control.