Skip to main content
. 2016 Aug 16;6:31607. doi: 10.1038/srep31607

Figure 7. Expression changes of key proteins involved in ROS mediated pathways.

Figure 7

(A) Expression analysis of p-NF-κB, MDM2, p53, p21 and PUMA in by241-treated MGC-803 cells. Western blot of protein extracted from MGC-803 cells following 24 h treatment with by241 (5 μM and 10 μM), a representative result of 3 independent experiments is shown. (B–F) Statistical analysis of p-NF-κB, MDM2, p53, p21 and PUMA proteins expression change induced by by241; (G) Expression analysis of p-PI3K, p-AKT (Thr308), p-AKT (Ser473), AKT, p-mTOR (Ser2448) and mTOR in by241-treated EC109 cells. (H–K) Statistical analysis of p-PI3K, p-AKT (Thr308), p-AKT (Ser473) and p-mTOR (Ser2448) proteins expression changes induced by by241; Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 were considered statistically significant compared with the controls.