FIG 4.

Histone chaperones Vps75 and MCM2 do not affect nucleosome disassembly by RSC. (A) Remodeling assays were performed using 20 nM dinucleosome substrate and 20 nM RSC (lanes 1 to 5), 20 nM RSC plus 300 nM Nap1 (lanes 6 to 10), and 20 nM RSC and 300 nM Vps75 (lanes 11 to 15). Samples were stopped at 0 and 40 s and 2, 6, and 20 min before being analyzed by gel shift. (B) Assays were performed as for panel A, except that they contained 20 nM RSC (lanes 1 to 6), 20 nM RSC plus 300 nM MCM2 (lanes 7 to 12), and 20 nM RSC plus 3 mM MCM2 (lanes 13 to 18). Samples were stopped at 0 and 20 s and 1, 3, 10, and 30 min.