FIG 4.
Analysis of the structural integrity of Gata3 YAC transgene by genomic qPCR. (A) Diagram of area around the Gata3 locus. PCR primer pairs for genomic quantitative PCR were designed at the five sites distributed from the 300-kb 5′ flanking sequence to the 130-kb 3′ flanking sequence in the Gata3 locus. The TgB125-G3 mouse line harbors a single copy of transgene for most of the part, while an additional 5′ end fragment may remain. (B) Whole-kidney sample of adult TgB125-G3 mice showed increased Gata3 mRNA expression in comparison with wild-type control (WT, n = 4; TgB125-G3, n = 5). The mRNA level is normalized with the GAPDH level. The statistical significance of the difference between WT and TgB125-G3 is indicated (*, P < 0.05; Student's unpaired t test). (C) Gross appearance of Gata3g/g::TgB125-G3 (G3YR) mouse and wild-type littermate. (D) Postnatal body weight gain of G3YR (n = 9) and wild-type (WT, n = 7) mice. Note that a certain fraction of G3YR mice show severe growth retardation.