TABLE 1.
Sequences of primers used in RT-qPCR and genomic qPCR
| Gene | Sense primer | Antisense primer | Assay |
|---|---|---|---|
| Kim1 | AAACCAGAGATTCCCACACG | GTCGTGGGTCTTCCTGTAGC | RT-qPCR |
| Ngal | CAAGCAATACTTCAAAATTACCCTGTA | GCAAAGCGGGTGAAACGTT | RT-qPCR |
| Nephrin | GACCGGGACACAAGAAGCTC | GATGTCCCCTCAGCTCGAAG | RT-qPCR |
| Podocin | CATGTGTCCAAAGCCATCCA | CTTTTCCCCTTCTGCAGCAA | RT-qPCR |
| Pdgfb | ATCCGCTCCTTTGATGATCT | GAGCTTTCCAACTCGACTCC | RT-qPCR |
| Pdgfra | TCCTTCTACCACCTCAGCGAG | CCGGATGGTCACTCTTTAGGAAG | RT-qPCR |
| Pdgfrb | CGGCCTGTGACTAGAAGTCC | GAGCTTGAGGCGTCTTGG | RT-qPCR |
| Megsin | GGCGGTTCAATTTGTCTACCA | ATGTACATGCTTATGCCACCA | RT-qPCR |
| Avpr2 | GGTCTCGGTCATCCAGTAGC | CTGGTGTCTACCACGTCTGC | RT-qPCR |
| Aqp2 | CAGCTCGAAGGAAGGAGACA | GCATTGGCACCCTGGTTCA | RT-qPCR |
| Gata3 | GGTGGACGTACTTTTTAACATCGA | CCCTGACGGAGTTTCCGTAG | RT-qPCR |
| Itih 5′-UTR | CTGGACAGGCTGGAATTGAT | CAGCGGGAGCTAACAAGAAC | Genomic qPCR |
| Itih 3′-UTR | AGATAGGGCATGTGGAGTGG | AGCTCGTGCTCATTGGAGAT | Genomic qPCR |
| Gata3 KE | GGCCTACTCTTTCTGGCAAA | AGCTGATGACCCAGTGAACC | Genomic qPCR |
| Int S1-E2 for YAC | CGAAGACCTAGTGCCCTCCAGAATTTCAGG | CTTGGCGCTCAGAGACGGTTGC | Genomic qPCR |
| Gata3 3′ 100 kb | AATGCTCCTCAAATGCATCC | TGTGTGTGTTTGCCAGGAAT | Genomic qPCR |