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. Author manuscript; available in PMC: 2016 Aug 16.
Published in final edited form as: Microbes Infect. 2009 Aug 9;11(14-15):1106–1113. doi: 10.1016/j.micinf.2009.08.004

FIGURE 1. Replication of T. gondii in human lymphoid tissue.

FIGURE 1

Tonsil blocks were infected with purified tachyzoites grown in the human fibroblastic cell line Hs27. Blocks were inoculated with various amounts of T. gondii ranging from 80 to 2000 tachyzoites per block. T. gondii replication was monitored by measuring concentration of p30 in culture medium of infected tissue blocks by ELISA (A). Panel (B) shows T. gondii replication in 16 different donors with the median and interquartile range at 12 days post infection. The replication of T gondii was confirmed by staining cells isolated from infected tissue blocks with Giemsa (C) or by staining with fluorescent anti-Toxoplasma antibodies (D).