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Effect of ethanol on caspase-3 and Bax activation in CHOP knockout mice. (A) The deletion of CHOP was verified by RT-PCR. (B) Postnatal day 7 CHOP knockout mice (CHOP−/−) and wild-type mice (CHOP+/+) were injected with ethanol as described. At specified times after ethanol injection, the brain was removed and the expression of active caspase-3 and Bax was analyzed with immunoblotting as described. The experiment was replicated 3 times. EtOH, ethanol.
