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. 2016 May 1;143(9):1585–1599. doi: 10.1242/dev.130591

Fig. 6.

Fig. 6.

Distribution of exocytic and endocytic factors in aura mutants. (A,B) The exocytic factor Rab11 properly localizes to the tips of microtubules at the furrow in wild-type (5/5) and aura mutant (6/6) embryos. (C,D) The exocytic factor Vamp2 localizes to mature furrows in wild type (5/5) but not in mutants (7/7). (E-H) The endocytic factors Clathrin and Dynamin 2 become enriched in ectopic CGs in aura mutants (F,F′,H,H′), whereas they exhibit a disperse cortical distribution in wild type (E,E′,G,G′). Clathrin appears localized to the CG surface in ectopic CGs in aura mutants (5/5 embryos, compared with 10/10 wild-type embryos with diffuse cortical labeling). Dynamin 2 also localizes to ectopic CGs (15/15 embryos, compared with 13/13 wild-type embryos with diffuse cortical labeling), which can appear as ring-like structures (3/15 embryos as shown in H′) or throughout the granule surface (12/15 embryos as shown in Fig. S5M,M′). Ectopic localization to CGs was confirmed by colabeling with WGA (not shown). (A′-H′) Higher magnifications of boxed regions in A-H. Scale bars: 100 μm in A-H; 10 μm in A′-H′.