Table 1.
Prevalence of crt and mdr-1 mutations in P. falciparum isolates from 2006–2007 to 2013–2014 along Grande Comore island
| Areas | Genes | Amino acid and genetic changesa | Number of isolates (%b) | |
|---|---|---|---|---|
| 2006–2007 (n = 115) | 2013–2014 (n = 87) | |||
| Mitsoudje center hospital | pfcrt | C72S (TGT → AGT) | 27 (23.5) | 3 (3.4)** |
| M74I (ATG → AAT) | 23 (20.0) | 5 (5.7)** | ||
| N75E (AAT → GAA) | 38 (33.0) | 6 (6.9)** | ||
| K76T (AAA → ACA) | 41 (35.7) | 5 (5.7)** | ||
| pfmdr-1 | N86Y (TGA → TGT) | 45 (39.1) | 25 (28.7) | |
| Y184F (ATA → ATA) | 25 (21.7) | 8 (9.2)** | ||
| D1246Y (GAG → GAT) | 12 (10.4) | 6 (6.9) | ||
| National Malaria Center | pfcrt | C72S (TGT → AGT) | 15 (13.0) | 2 (2.3)** |
| M74I (ATG → AAT) | 13 (11.3) | 4 (4.6) | ||
| N75E (AAT → GAA) | 19 (16.5) | 6 (6.9)* | ||
| K76T (AAA → ACA) | 27 (23.5) | 8 (9.2)** | ||
| pfmdr-1 | N86Y (TGA → TGT) | 28 (24.3) | 20 (23.0) | |
| Y184F (ATA → ATA) | 21 (18.3) | 12 (13.8) | ||
| D1246Y (GAG → GAT) | 10 (8.7) | 2 (2.3) | ||
| Mitsamiouli Center Hospital | pfcrt | C72S (TGT → AGT) | 7 (6.1) | 1 (1.1) |
| M74I (ATG → AAT) | 9 (7.8) | 4 (4.6) | ||
| N75E (AAT → GAA) | 16 (13.9) | 4 (4.6)* | ||
| K76T (AAA → ACA) | 15 (13.0) | 4 (4.6)* | ||
| pfmdr-1 | N86Y (TGA → TGT) | 27 (23.5) | 8 (9.2)** | |
| Y184F (ATA → ATA) | 14 (12.2) | 6 (6.9) | ||
| D1246Y (GAG → GAT) | 8 (6.8) | 4 (4.6) | ||
| All the examined sites | pfcrt | C72S (TGT → AGT) | 49 (42.6) | 6 (6.9)** |
| M74I (ATG → AAT) | 45 (39.1) | 13 (14.9)** | ||
| N75E (AAT → GAA) | 73 (63.5) | 16 (18.3)** | ||
| K76T (AAA → ACA) | 83 (72.2) | 17 (19.5)** | ||
| pfmdr-1 | N86Y (TGA → TGT) | 100 (87.0) | 35 (40.2)** | |
| Y184F (ATA → ATA) | 60 (52.2) | 26 (29.9)** | ||
| D1246Y (GAG → GAT) | 30 (26.1) | 12 (13.8)* | ||
aThe mutated amino acids and nucleotides are indicated in bold type
bStatistically significant differences for comparison with isolates circulating in 2006–2007 from Grande Comore island (* P < 0.05; ** P < 0.01) using Mann–Whitney U test