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. 2016 Aug 15;14:44. doi: 10.1186/s12958-016-0176-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Cytokeratin immunostaining of cultured BOEC in different number of cell culture passages. Immunostaining with anti-cytokeratin antibody of cultured BOEC in: a passage 0; b passage 1; c passage 2; d passage 3; and e negative control. Goat anti-mouse-IgG DyLight 488 conjugate (green) was used for staining cytokeratin as a secondary antibody. DAPI (blue) was used to visualize nuclei. Magnification was set at 200 X. Bar in each figure represents 100 μm. Representative pictures of BOEC cultured in LOW glucose medium are shown