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. 2016 Aug 15;14:44. doi: 10.1186/s12958-016-0176-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Effect of passage number or estrous cycle stage on enzymes of cellular metabolism mRNA expression. Normalized mRNA expression of a BDH2; and d OXCT2 in BOEC of in vivo samples and of cell culture passages P0, P1, P2 and P3 with LOW and HIGH glucose medium content (n = 8); and e magnified inset of OXCT2 in BOEC in cell culture passages P0, P1, P2 and P3 with LOW and HIGH glucose medium content as well as normalized mRNA expression depending of the phase of the estrous cycle on day of collecting cells of b BDH2 in LOW glucose medium; c BDH2 in HIGH glucose medium; f OXCT2 in LOW glucose medium; and g OXCT2 in HIGH glucose medium (n = 4). Different letters indicate significant difference (P < 0.05) between cell culture passages of the same glucose medium content (ab and de for LOW and HIGH glucose medium content, respectively). Asterisk on top of the line represents significant difference between the different estrous cycle phases on day of collection (P < 0.05)