Fig. 7.

Effect of passage number or estrous cycle stage on GAPDH, SDHA and HK1 mRNA expression. Normalized mRNA expression of a GAPDH; d SDHA; and g HK1 in BOEC of in vivo samples and of cell culture passages P0, P1, P2 and P3 with LOW and HIGH glucose medium content (n = 8) as well as normalized mRNA expression depending of the phase of the estrous cycle on day of collecting cells of b GAPDH in LOW glucose medium; c GAPDH in HIGH glucose medium; e SDHA in LOW glucose medium; and f SDHA in HIGH glucose medium (n = 4). Different letters indicate significant difference (P < 0.05) between cell culture passages of the same glucose medium content. Asterisk on top of the line represents significant difference between different glucose content of medium at each cell culture passage or between the different estrous cycle phases on day of collection (P < 0.05)