Table 2.

Primer pairs for amplification of reference genes

Gene	Sequence of nucleotide	Accession no. /Reference	Product size (bp)	Tm (°C)
HDAC1	for 5′ –CCA GTG CAG TTG TCT TGC AG- 3′	NM_001037444.2	217	60
	rev 5′ –TTA GGG ATC TCC GTG TCC AG- 3′
UXT	for 5′ –CGC TAC GAG GCT TTC ATC TC- 3′	NM_001037471.2	207	61
	rev 5′ –TGA AGT GTC TGG GAC CAC TG- 3′
PPIA	for 5′ – CTG AGC ACT GGA GAG AAA GG- 3′	NM_178320.2	259	60
	rev 5′ – TGC CAT CCA ACC ACT CAG TC- 3′
RPL19	for 5′ -GGC AGG CAT ATG GGT ATA GG- 3′	NM_001040516.1	232	60
	rev 5′ -CCT TGT CTG CCT TCA GCT TG- 3′
SUZ12	for 5′ -TTC GTT GGA CAG GAG AGA CC- 3′	[59]	286	60
	rev 5′ -GTG CAC CAA GGG CAA TGT AG- 3′

Selected gene transcripts, primer sequences and annealing temperatures (Tm) used for normalization of quantitative PCR with resulting amplicon length